THE CNR MICROSCOPY PLATFORM - NIKON REFERENCE CENTER - VIA DEGLI APULI LABORATORIES

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Nikone Center
Tuesday, 9 December, 2014

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ISTITUTO DI BIOLOGIA, MEDICINA MOLECOLARE E NANOBIOTECNOLOGIE- CNR

The laboratories of CNR-IBMN(Institute of Biology, Molecular Medicine and Nanobiotechnologies) @ San Lorenzo host the Nikon Reference Center for Central-Southern Italy,  with last-generation widefieldlight microscopy equipment and advanced imaging software for  automated image analyses. Imaging capabilities include:

•Time-lapse recording of live cells over several days,
• Detecting intracellular molecular interactions,
• Reconstructing cellular and subcellular structures at 3D level,
• Obtaining quantitative information on the molecules or cells that are being imaged.
•The Center is open to external users.
Management: Giulia Guarguaglini
                      Patrizia Lavia
Intranet site to check/book available microscopes:
http://aulegest.uniroma1.it/aulegest1/fac14/home.php
Contact: Giulia Guarguaglini
              Lia Asteriti
Our equipment
Up-right microscope delivering crisp, clean and
true-to-life images of fixed fluorescent cell sample.
Inverted microscope for
time-lapse  imaging of living cells.
90i
ti
Nikon Eclipse 90i microcospe
           Nikon Eclipse Ti microscope
Workstation with Nis-Elements HC 4.20 software (Nikon) for qualitative (deconvolution, 3D-projection, Extended Depth
of Focus, LUTs, etc) and quantitative image processing. http://www.nis-elements.com/

FIELDS OF APPLICATION

The IBMN-Nikon microscopy platform supports versatile applications for both fixed and live cell samples and provides opportunities for coupling dynamic studies (time-lapse recording) with high resolution qualitative and quantitative image analysis of cells and cellular structures. Applications and scopes include studies of:

•Dynamic processes, e.g. signaling, intracellular transport, organization of organelles and  subcellular structures
•Cell division and checkpoints
•Inhibition of tumor cell growth
•Differentiation of stem and progenitor cells
•Validation of innovative drugs
•The cell response to parasites, bacteria and viral infectious agents
•The induction of different cell death pathways in response to  particular stimuli, damage and stress conditions
•Novel biocompatible matrices to support cell growth in studies of tissue regeneration
•The interaction of nanoparticles with cells in toxicity and therapeutic studies
•The interaction between proteins and molecules at the intracellular site(s) at which they take place
MORE ON APPLICATIONS...

Downloads and useful sites:
Instruments features:


General rules
Eclipse 90i 
Eclipse Ti  






 

Brochures:


90i
Ti
NIS-Elements software    
OKOLab incubation system
90i camera
Ti camera




 

Useful microscopy sites:


http://www.microscopyu.com (microscopy tutorial)
http://www.olympusmicro.com (microscopy tutorial)
http://zeiss-campus.magnet.fsu.edu (microscopy tutorial)
http://learn.hamamatsu.com (digital imaging)
http://micro.magnet.fsu.edu (microscopy tutorial)
http://cellimages.ascb.org (image library)
http://www.openmicroscopy.org/site/products/omero (open acces image organiser and analyser)
http://www.eurobioimaging.eu/ (pan‐European research infrastructure project)


We are grateful to Assicurazioni Generali Spa, Fondazione Roma Terzo Settore and Fondazione Monte dei Paschi di Siena for their financial support in the set-up of the microscopy lab.